Elisa Reader And Washer Principle

The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically.

Elisa reader and washer principle.

In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates. The elisa reader reads the plate. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present.

Elisa is a plate based assay technique. The illustration below shows a workflow for a typical sandwich elisa assay. An immune reaction i e. The enzyme activity is measured using a substrate that changes color when modified by the enzyme.

This ability to wash away non specifically bound materials makes the elisa a powerful tool for measuring specific analytes within a crude preparation. Elisa stands for enzyme linked immunosorbent assay. The test is done in an elisa plate also known as a 96 well plate or microplate. And a plate reader software is used to plot standard curves and calculate results.

Are available as fixed as well as adjustable volume as well as single channel and multi channel. What an elisa reader does.